Figure 5.

Effects of siRNAs against HIF-1α or erythropoietin (Epo) on cell proliferation under (A) normoxia or (B) hypoxia in SKRC44 cells. SKRC44 cells were treated overnight with buffer control, negative control and siRNAs against HIF-1α or Epo, and cultured for 48 h. The cells were cultured for an 12 h under normoxia or hypoxia. SKRC44 cells treated with buffer control under normoxia were used as control. An siRNA against Epo inhibited growth of SKRC44 in both hypoxia and normoxia. An siRNA against Hif-1α did not inhibit growth of SKRC44. The proliferation rate was measured using the WST-1 assay. HIF, hypoxia-inducible factor; siRNA, small interfering RNA.