Fig. 1.
Representative HPLC-FL chromatograms of a urophysial sample of round goby and b the same sample spiked with standard AVT (6.6 pmol/mL) and IT (6.8 pmol/mL). Chromatographic conditions: Agilent Zorbax Eclipse XDB-C18 column (150 mm × 4.6 mm I.D., 5 μm particle); elution: solvent A (0.1 % TFA in H2O), solvent B [0.1 % TFA in acetonitrile : H2O (3:1)], linear gradient 45–80 % of eluent B in 12 min; flow rate 1 mL/min; column temperature 20 °C; injection volume 40 μL; detection: FL, excitation 470 nm, emission 530 nm