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Summary steps for production of GeMI-Vax cells.
| Time (min) | OD600 | Temperature |
Induction |
Description | ||
|---|---|---|---|---|---|---|
| E. coli (°C) | Shigella (°C) | E. coli | Shigella | |||
| 0 | 0.3 | 30 | 30 | IPTG | IPTG | Induce malaria Ag expression |
| 60 | 0.6 | 30 | 40 | Arabinose | Temp shift | Induction inactivation gene expression |
| 150 | 30 | 40 | – | – | Final inactivation with streptomycin | |
| 270 | 30 | 40 | – | – | Harvest and wash cells | |
| 48 h | 30 | 40 | – | – | Lyophilization | |
