Figure 1.

Gating scheme used for polychromatic flow cytometry of HIV Gag-specific T-cell responses in the female genital tract and blood. Cells were first gated on singlets, live cells and then lymphocytes. From this gate, CD3⁺ cytokine-producing T cells were gated, and then further gated on CD4⁺ and CD8⁺ T cells. Gates for each of the four functions were set based on fluorescence minus one and the negative control and were kept constant for all samples analysed. Data analysis was performed using FlowJo v8 8 6.