Table 1.

Primer sequences used for amplification of CRTH2, cyclo-oxygenase 2 (COX-2), L-19 and the cytokines: interleukin-4 (IL-4), IL-10, interferon-γ (IFN-γ) and tumour necrosis factor-α (TNF-α)

	Forward 5′–3′	Reverse 5′–3′	bp
CRTH2	CGTAGCCGTGAGCCTGCGACTG	GGCGATTGCGGAGCCCACCACT	344
COX-2	AGCCAGGCAGCAAATCCTTGCTGTT	TCAAATCCTGTGCTCATACATTCC	71
IFN-γ	AGCGGCTGACTGAACTCAGATTGTAG	CGGGTGTAGTCACAGTTTTCAGCTGTATAGG	252
TNF-α	CATCTTCTCAAAATTCGAGTGACAA	TGGGAGTAGACAAGGTACAACCC	175
IL-4	CGAAGAACACCACAGAGAGTGAGCT	GACTCATTCATGGTGCAGCTTATCG	237
IL-10	ACCTGGTAGAAGTGATGCCCCAGGCA	CTATGCAGTTGATGAAGATGTCAAA	181
L-19	GAAAAAGAAGGTCTGGTTGGA	TGATCTGCTGACGGGAGTTG	72