Figure 2.

n-Butyrate modulates expression of genes from the canonical eicosanoid pathway. Freshly isolated monocytes were incubated with lipopolysaccharide (LPS; 100 ng/ml) in the presence or absence of n-butyrate (1 mm) for 6 hr. Expression profiles of 19 genes of the eicosanoid pathway were analysed using the ΔΔCT method for relative quantification. Expression levels were normalized to the average of housekeeping genes and shown relative to unstimulated cells in the absence of n-butyrate. Colour code: light grey, n-butyrate versus control; black, LPS versus control, dark grey LPS + n-butyrate versus control. Data are representative of two independent experiments. For better overview, analysed genes were divided into (a) leukotriene-related and (b) prostaglandin-associated genes.