Figure 4.
Prostaglandin release by monocytes after bacterial stimulation in response to n-butyrate. Monocytes were stimulated with (a) lipopolysaccharide (LPS; 100 ng/ml) or (b) Staphylococcus aureus cells (SAC; 7·5 μg/ml) and co-incubated with n-butyrate (1 mm) for varying times, and levels of prostaglandins (PGE2 and 15d-PGJ2) were determined in culture supernatants by ELISA. Results are expressed as mean ± SEM of five independent experiments each performed in duplicate. * P ≤ 0·05 (paired Student's t-test)