Figure 1.
Cardiomyocyte-specific ablation of Mcl-1 in the adult heart leads to contractile dysfunction and death. (A) Western blot analysis of MCL-1 in control hearts and in the border zone of the infarct. (B) Quantitation of outer mitochondrial membrane (OMM) and matrix MCL-1 isoforms (n = 5–6). (C) Western blot analysis for MCL-1 in heart and skeletal muscle after 2 wk. (D) Representative M-Mode echocardiograms at 2 wk. (E) Echocardiographic analysis revealed reduced ejection fraction (EF) and fractional shortening (FS) as well as enlarged left ventricular diastolic dimension (LVDd) in Mcl-1f/fCre+ mice. n = 5–14; (*) P < 0.05. (F) Kaplan-Meyer survival curve (P < 0.05). Data are mean ± standard error of the mean (SEM).