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Expression of STAT3 during preimplantation mouse development. (A) Immunodetection with pan-STAT3 antibody indicates nuclear localization of STAT3 starting from the four-cell stage. (B) Immunodetection with antibody specific to phosphorylated tyrosine (pY705)-STAT3 and OCT4 show nuclear STAT3 is tyrosine-phosphorylated and correlates with re-expression of OCT4 in the four-cell embryos. Bar, 20 μm.
