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. 2013 Mar 27;6(4):925–933. doi: 10.1242/dmm.011494

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© 2013. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 3. — Increased mTORC1 signaling in tumors arising from tsc2;p53 compound mutant zebrafish compared with p53^zdf1/zdf1 zebrafish. (A) Immunoblotting of a series of abdominal tumors comparing p53^zdf1/zdf1 mutant zebrafish (lanes marked Tsc2^+/+) and tsc2;p53 compound mutants (lanes marked Tsc2^+/−). (B) Quantification of phospho-S6 levels were normalized to actin expression (P=0.25). (C) Quantification of total S6 levels were normalized to actin expression (P=0.06). (D) Increased ratio of normalized phospho-S6 to total S6 levels in tsc2;p53 compound mutant compared with p53^zdf1/zdf1 zebrafish; asterisk denotes significance by two-tailed Student’s t-test, P=0.03. n=5 for p53 mutant and n=7 for compound mutant zebrafish, although two tumor samples from tsc2;p53 compound mutant zebrafish (lanes 4,5 and 11,12) were divided in half and processed independently for immunoblotting.