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. 2013 Mar 27;6(4):925–933. doi: 10.1242/dmm.011494

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© 2013. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 4. — AKT activity in p53^zdf1/zdf1 and tsc2;p53 compound mutant tumors. (A–F) Phospho-tuberin (p-Tsc2) staining in p53^zdf1/zdf1 and tsc2;p53 compound mutant zebrafish. (B,C and E,F) Higher magnification of A and D, respectively. (C,F) Phospho-tuberin (Ser939, red); (B,E) phospho-tuberin (red) merged with DAPI (blue). (G,J) Total Akt immunofluorescence, (H,K) phospho-Akt (Thr308) immunofluorescence and (I,L) phospho-Akt (Ser473) immunofluorescence in p53^zdf1/zdf1 (G–I) and tsc2;p53 compound mutant (J–L) zebrafish. M, muscle; T, tumor. Scale bars: 100 μm.