Figure 1.

Preparation of serial thin sections. (a) Block face with parallel top and bottom edges. (b) Carbon coated coverslip submerged in Jumbo Histo knife. Use eyelash tool to detach ribbon (arrow) from knife-edge (1) and move it to attach to metal bar holding water in place (2) then slowly and carefully remove the coversip from boat so ribbon lays down flat. (c) FESEM image of ribbon of ultrathin serial sections mounted on carbon-coated coverslip. Scale bar 100 μm. Animal use in this experiment was conducted in strict accordance to our institutional animal care and use committee guidelines.