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. 2013 May 20;98(7):E1152–E1162. doi: 10.1210/jc.2013-1081

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Copyright © 2013 by The Endocrine Society

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Figure 3. — Cellular impact of NR2F2 knockdown. Cells were transfected with siRNA to NR2F2 (siNR2F2) or nontargeting siRNA (siNT) as indicated. After knockdown was confirmed (Supplemental Figure 3) (A and B), proliferation was measured in either ES-2 (A) or TOV-112D (B) cells transfected with either siNR2F2 or siNT (n = 3). Results are standardized to day 2. C and D, Apoptosis was measured in either serum-starved cultures of either ES-2 (C) or TOV-112D (D) cells transfected with either siNR2F2 or siNT (n = 3). Caspase-3/7 is reported in relative fluorescence units. *, P < .05; **, P < .01.