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Selective enrichment of O-GlcNAcylated and phosphorylated synthetic peptides in a synaptosome sample via BEMAD followed by thiol affinity chromatography. (a) LC/MS trace of d0-DTT-conjugated phosphorylated peptide. (b) LC/MS trace of d6-DTT-conjugated O-GlcNAcylated peptide. (c) MS trace of singly charged d0-DTT-labeled phosphorylated peptide (M+H, 1051.4) and d6-DTT-labeled O-GlcNAcylated peptide (M+H, 1057.4).
