Table 1.

Oligonucleotide sequences of PCR primers

Name	Sequence
A
VYWFR-SfiIB	5′-TGGCCGAGGCGGCCCNCGRAACCARTANAC
VYWF-SfiIB	5′-GACTGGCCGAGGCGGCCCRAACCARTANAC
YWFK-SfiIB	5′-GACTGGCCGAGGCGGCCCYTTRAACCARTA
YWFR-SfiIB	5′-GACTGGCCGAGGCGGCCCNCGRAACCARTA
WFK-SfiIB	5′-GACTGGCCGAGGCGGCCCYTTRAACCA
WFR1-SfiIB	5′-GACTGGCCGAGGCGGCCCNCGRAACCA
WFR2-SfiIB	5′-GACTGGCCGAGGCGGCCCYCTRAACCA
B
MDIR1-IgD-F*	5′-GACTGGCCATTATGGCCTAAGTGGACCTCGTGCAG
MDIR1-IgD-R*	5′-GACTGGCCACCGCGGCCGTTAGTATTATGTTTCCA
MDIR2-IgD1-F*	5′-GACTGGCCATTATGGCCTGTGGGGGAAGGACCATG
MDIR2-IgD1-R	5′-AGACGCAGCTTCATCAGAGACTTG
MDIR2-IgD2-F	5′-GTTCCTGTGCTTGGATTACTGTCA
MDIR2-IgD2-R*	5′-GACTGGCCACCGCGGCCAAGTGTCCTTGGTGTGAA
MDIR4-IgD2-F	5′-CTACTCCTGTGCTCAGATATCTGTC
MDIR4-IgD2-R	5′-ACGTTCCTGCTCCTTTCAAAAC
TypeIIcon-F1	5′-GTTTCAGGTGCANTGTGGGSRRAG
TypeIIcon-R1	5′-TCCACAKCYGTACCAYCCTGNATC
TypeIIcon-R2	5′-ATCCWCCAYAGTCACAGTWA
TypeIIIcon-F1	5′-CCAACCAATGTCTCCTGTCTCGG
TypeIIIcon-R1	5′-CACGTTGACCAATTTACTGGATTT
TypeIIIcon-R2	5′-CACGTTGACCATTTCACTGGATTT

A Amptrap, B MDIRs. MDIR-specific primers marked with an asterisk contain SfiI restriction sites at their 5′ ends