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Summary of Kinetic Parameters on Human MCD
| Enzyme | Km (μM) | kcat (s−1) | kcat/Km (M−1s−1) |
|---|---|---|---|
| Wild-type HsMCD | 38 ± 12 | 33 ± 2 (1)a | 8.7 × 105 (1) |
| Quintuple SER mutant | 58 ± 17 | 45 ± 4 (0.73) | 7.8 × 105 (1.1) |
| H423N | 32 ± 4 | 4.7 ± 0.1 (7.0) | 1.4 × 105 (6.2) |
| S329A | 19 ± 4 | 0.30 ± 0.01 (110) | 1.5 × 104 (58) |
| Y456S | 132 ± 19 | 44 ± 2 (0.75) | 3.3 × 105 (2.6) |
| S290F | 37 ± 5 | 15 ± 1 (2.2) | 4.1 × 105 (2.1) |
The ratio for values between the wild-type and mutant enzymes are given in the parentheses.
