Figure 3.

Early T and B Cell Progenitors Are Suppressed and Myeloid Biased in Flt3^ITD/ITD Mice

(A) Thymic cellularity of Flt3^+/+ and Flt3^ITD/ITD mice at 2–3 and 8–10 weeks (n = 7–10 mice of each genotype at each age).

(B) Representative DN staging of Flt3^+/+ and Flt3^ITD/ITD thymocytes from 2-week-old mice; percentages are relative to total thymocytes.

(C) Progressive reduction of DN1Kit⁺ cells in Flt3^ITD/ITD thymus.

(D) Expression (SEM) of Ccr9 in LSKCD48⁺150⁻ BM cells from 8- to 10-week-old Flt3^+/+ and Flt3^ITD/ITD mice (n = 3–4 mice per genotype, 2–3 separately sorted replicates per mouse).

(E) T cell potential on OP9DL1 cells of DN1 cells (10/cells per well) from 2- to 3-week-old Flt3^+/+ and Flt3^ITD/ITD mice (n = 2 mice per genotype, 12 replicate wells per mouse). ND, not detected.

(F) Representative staging of early B cell progenitors in Flt3^+/+ and Flt3^ITD/ITD 2- to 3-week-old mice. pre-pro-B cells are identified as Lin⁻B220⁺CD19⁻CD24⁻AA4.1⁺CD43^mid; percentage of total BM cells.

(G) Increased numbers of pre-pro-B cells in 2- to 3-week-old Flt3^ITD/ITD mice (mean [SEM] values for 2- to 3-week-old mice, n = 8–10 of each genotype).

(H) Reduced numbers of pro-B cells (Lin⁻B220⁺CD43⁺CD19⁺CD24^intAA4.1⁺) in Flt3^ITD/ITD mice.

(I) Mean (SEM) expression of lymphoid-affiliated genes in pre-pro-B cells from 2- to 3-week-old mice (n = 2 mice per genotype, 3 separately sorted replicates per mouse).

(J) Mean (SEM) expression of myeloid-affiliated genes in pre-pro-B cells from Flt3^ITD/ITD mice.

(K) In vitro B cell potential (B220⁺CD19⁺ cells) of pre-pro-B cells from 2- to 3-week-old mice (n = 10 cells/well, 2 mice per genotype, 10 replicate wells per mouse).

Error bars represent SEM. See also Figure S3.