Figure S1.

Mutation of Runx1 in Flt3^ITD/ITD Mice Results in High-Penetrance Aggressive Myeloid Leukemia, Related to Figure 1

(A–C) Hematologic indices in Flt3^ITD/ITDRunx1^fl/flMx1Cre⁺ leukemic mice demonstrating a marked increase in white blood cell count (WBC) (A), anemia (B), and thrombocytopenia (C) compared with Flt3^ITD/ITDRunx1^fl/flMx1Cre⁻ controls. Mean (SEM) values from 6-14 mice from 5-11 independent experiments. Hematologic indices were measured using a Sysmex^® automated analyzer.

(D) Representative FACS analysis of infiltrated livers and spleens from Flt3^ITD/ITDRunx1^fl/flMx1Cre⁺ leukemic mice showing lineage surface markers typical of myeloid leukemic cells (results representative of 3 [liver] and 11 [spleen] further analyzed cases).

(E) Recombination at the Runx1 locus without poly I:C induction in Flt3^ITD/ITDRunx1^fl/flMx1Cre⁺ leukemic BM. Representative PCR analysis of Runx1^fl/fl recombination in paired BM and earclip (EC) DNA from Flt3^ITD/ITDRunx1^fl/flMx1Cre⁺ leukemic and Flt3^ITD/ITDRunx1^fl/flMx1Cre⁻ and Flt3^+/+Runx1^fl/flMx1Cre⁺ controls, all without polyI:C induction. The upper band represents non-deleted Runx1 allele and the lower band indicates deleted Runx1 alleles. Results are representative of 3 further analyzed leukemic samples. Error bars represent SEM.