Figure S4.

Involvement of Pu1 Upregulation in the Myeloid Bias of Flt3^ITD/ITD Progenitors, Related to Figure 4

(A) Gene set enrichment analysis demonstrating upregulation of Pu1 target genes in Lin⁻CD34⁺CD38⁻CD90⁻CD45RA⁺ cells from FLT3-ITD mutated human AML (n = 8) relative to FLT3-WT counterparts (n = 18).

(B) Representative FACS analysis of Pu1-YFP expression levels during early B cell commitment demonstrating marked downregulation of Pu1 expression at the pre-pro-B to pro-B transition. Representative of 2 experiments.

(C) Western blot showing increased pStat3 activation in the BM of Flt3^ITD/ITD mice in comparison with Flt3^+/+ BM. The blot shown is representative of two independent experiments.