Table 4.
Capacity of the (H6-ED3-PhoA)2 reagents to discriminate between DENV-infected and DENV-uninfected serums in a MAC-ELISA
| (+) Serums | R1 | R2 | R3 | R4 |
|---|---|---|---|---|
| DENV1 |
0.94 ± 0.05 |
0.83 ± 0.06 |
0.64 ± 0.08 |
0.59 ± 0.08 |
| DENV2 |
0.90 ± 0.03 |
0.88 ± 0.05 |
0.57 ± 0.06 |
0.42 ± 0.07 |
| DENV3 |
0.82 ± 0.06 |
0.75 ± 0.07 |
0.77 ± 0.07 |
0.18 ± 0.08 |
| DENV4 |
0.73 ± 0.05 |
0.55 ± 0.06 |
0.45 ± 0.06 |
0.61 ± 0.07 |
| DENV1-4 | 0.83 ± 0.03 | 0.73 ± 0.04 | 0.59 ± 0.04 | 0.47 ± 0.04 |
R1 to R4, (H6-ED3-PhoA)2 dimers of serotypes DENV1 to DENV4, respectively. The first column gives the serotype of the infecting DENV for the (+) serums. DENV1-4, the serotype of the infecting virus could be any one of DENV1 to DENV4. The DENV-uninfected serums were always taken as (−) serums. Each entry gives the accuracy and associated standard error of a MAC-ELISA that used the Ri reagent (i = 1, …, 4) as an antigen and was evaluated on the (+) and (−) serums.