Table 5.

Capacity of the (H6-ED3-PhoA)₂ reagents to discriminate between serums infected by different DENV serotypes in a MAC-ELISA

(−) Serums	R1	R2	R3	R4
DENV1	na	0.52 ± 0.09	0.55 ± 0.10	0.57 ± 0.08
DENV2	0.78 ± 0.08	na	0.68 ± 0.08	0.70 ± 0.07
DENV3	0.84 ± 0.07	0.72 ± 0.08	na	0.80 ± 0.07
DENV4	0.87 ± 0.06	0.85 ± 0.05	0.81 ± 0.07	na
Other DENV	0.84 ± 0.06	0.72 ± 0.06	0.70 ± 0.06	0.69 ± 0.06
All others	0.90 ± 0.05	0.81 ± 0.04	0.73 ± 0.06	0.66 ± 0.06

R1 to R4, (H6-ED3-PhoA)₂ dimers of serotypes DENV1 to DENV4, respectively; na, non applicable. The serotype of the infecting DENV for the (+) serums was always identical with the serotype of the reagent. The first column gives the serotype of the infecting DENV for the (−) serums. Other DENV, all DENV serotypes except the serotype of the reagent. All others, all serums (infected and uninfected) except those infected by the same DENV serotype as the reagent. Each entry gives the accuracy and associated standard error of a MAC-ELISA that used the R_i reagent (i = 1, …, 4) as an antigen and was evaluated on the (+) and (−) serums. Note that the expressions (+) serums and (−) serums refer to the discrimination under statistical analysis and not to the infected or uninfected character of the serums.