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. 2013 Jul 4;8(7):e67693. doi: 10.1371/journal.pone.0067693

Figure 9. HMGB-1 blockade reduces NTS activity by limiting macrophage infiltration of the kidney of Lcn-2 KO mice.

Figure 9

WT mice (black bar; n = 5), Lcn-2 KO mice treated with an isotype control antibody (white bar; n = 6) or with an anti-HMGB-1 antibody (grey bar; n = 5). Seven days after disease induction (A) staining for Gr-1+ cells, CD68+ and CD8+ cells was performed. The number of positive pixels in 6 analysis fields is given. (B) Kidney sections were stained for infiltration by CD4+ T cells. The number of positive cells in 6 high power fields is given. (C) cDNA isolated from kidney samples was analysed for the expression of the respective genes. The ratio of the respective gene to the housekeeping gene HPRT is provided. (D) Scoring for PAS-positive deposits was performed. All data are given as mean ± SEM. Af = analysis field; Hpf = high power field; *p<0.05 and **p<0.01 WT compared to Lcn-2KO mice+isotype. #p<0.05 and ##p<0.01 Lcn-2KO mice+isotype compared to Lcn-2KO mice+a-HMGB-1 Ab.