Table 1. Sequences of PCR primers used for real-time PCR of cell culture experiments.

Gene of interest	Forward Primer	Reverse Primer
TLR1	5′-ggacctacccttgcaaacaa-3	5′-ggtggcacaagatcaccttt-3
TLR2	5′-ttatcttgcgcagtttgcag-3	5′-ctcccacttcaggctctttg-3
TLR3	5′-agcatcaaaagaagccgaaa-3	5′-cttgctgaactgcgtgatgt-3
TLR4	5′-gctcctggctaggactctga-3	5′-tgtcatcagggactttgctg-3
TLR5	5′-ctggggacccagtatgctaa-3	5′-acagccgaagttccaagaga-3
TLR6	5′-acacaatcggttgcaaaaca-3	5′-ggaaagtcagcttcgtcagg-3
TLR7	5′-attccttgcctcctgaggtt-3	5′-gctgaggtccaaaatttcca-3
TLR8	5′-agtttgcacattccctggac-3	5′-agaggaagccagagggtagg-3
TLR9	5′-actgagcaccctgcttcta-3	5′-ggctcaggctaagacactgg-3
Lcn-2	5′-gcctcaaggacgacaacatca-3	5′-caccacccattcagttgtcaat-3
IL6	5′-cagaggataccactcccaaca-3	5′-ttctgcaagtgcatcatcgt-3
TNF-α	5′-cagttggtggtttgctacga-3	5′-ccccaaagggatgagaagtt-3