Figure 5.

Temperature stability (A) and pH activity (B) profiles of CvChi45. (A) The recombinant protein (150 ng/μL) was incubated for 30 min at varying temperatures and then centrifuged (10,000 g, 10 min, 4°C), and the residual chitinolytic activity was determined in the supernatant as described in the methods section, using colloidal chitin as a substrate. (B) Samples of recombinant CvChi45 (150 ng/μL) were dialyzed for 1 h against buffers with different pH values, and the hydrolytic activity was determined under standard assay conditions using colloidal chitin as a substrate. The relative activity was expressed as a percentage of the highest activity recorded at a certain temperature or pH, respectively.