Figure 1. Effect of the duration of acetic acid treatment on AMPKα phosphorylation and activity in hepatocytes.

Hepatocytes were treated with 3.6 mM acetate (neutralized acetic acid) for 0, 1, 3, 6, 10 16, and 24 h. A: Western blotting results of p-AMPKα and AMPKα. B: The phosphorylation level of AMPKα (p-AMPKα/AMPKα). C: AMPKα activity. * p<0.05, ** p<0.01 versus the control group.