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. 2013 Jul 4;8(7):e67811. doi: 10.1371/journal.pone.0067811

Figure 3. Gene expression analysis of Wnt signalling in monolayer (Mono) and anoikis resistant (AR) cells of normal breast cell lines (N), ER-ve and ER+ve breast cancer cell lines.

Figure 3

A) Protein expression of activated B-catenin (unphosphorylated), Lef1, Axin2, DKK1 and B-actin (housekeeper) in MCF7 monolayer and AR cells. B) Cluster analysis was performed using the fold change in expression between Mono and AR cells. Data is displayed in a heatmap represented by either decreased (green) or increased (red) expression compared to the mean mRNA expression. (Red) ↑↑ Indicates significant increased expression (<0.05) (red) ↑ indicates a trend towards increased expression. (Green) Indicates significant increased expression (<0.05) (green) indicated a trend towards increased expression.