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. 2013 Jul 4;8(7):e67840. doi: 10.1371/journal.pone.0067840

Figure 3. YukE production and secretion by B. subtilis strains ATCC 6051 (WT) and 168 and by their derivatives carrying mutations in deg genes.

Figure 3

A. Growth curve of WT strain in LB at 37°C. A fitting curve for the exponential growth phase is presented. Growth curves of deg strains showed no significant differences (not shown). The arrows indicate the time points where culture samples were collected for production of protein extracts. T0, end of exponential growth phase; T2, 2 hours after entry in stationary growth phase. B. Immunodetection of YukE and TrxA (cytosolic control protein) in supernatant precipitates (SN) and cell extracts (C) prepared from T0 and/or T2 culture samples of strain ATCC 6051 (WT) and its derivatives W654 (degU32(Hy)), WTF28 (degU) and W648 (degS). Each lane was loaded with 20 µg total protein, except that of pure YukE-His6 (100 ng). C. Immunodetection of YukE and TrxA in SN and C fractions prepared from T0 and/or T2 culture samples of strain 168 and its degU32(Hy)) derivative. The loaded protein amounts were as in panel B, except for the LB precipitate control that was with a volume equivalent to the maximum loaded SN fraction. The corresponding Coomassie blue stained gels of SN and C extracts are provided as Figure S1.