Figure 5. YukE production and secretion by BsEss mutant strains.
A. YukE and TrxA (cytosolic control protein) were immunodetected in supernatant precipitates (SN) and cell protein extracts (C) of strain W654 (degU32(Hy)) and its derivatives with individual knockouts of BsEss genes. Note that the YukE signal shown for cell extracts results from an overexposed film when compared to that of SN fractions. Immunodetection of YueB polypeptides is also shown for cell extracts. Each lane was loaded with 20 µg total protein, except that of the control that was loaded with 100 ng of pure YukE-His6. B. Immunodetection of YukE and TrxA in SN and C fractions of the yueB complementation strain, in absence or presence of 0.5% xylose. The immunodetection of YueB polypeptides and the amount of loaded protein was as in panel B. The corresponding Coomassie blue stained gels of SN and C extracts are provided as Figure S2.