Figure 5. The effect of STAT3α M206K mutation on the sensitivity of DLBCL cells to JAK2 inhibitor TG101348.

(A–B) The effect of JAK2 inhibitor TG101348 on STAT3 dephosphorylation was assessed in WT STAT3α^WT and mutant STAT3α^M206K in stably transfected HEK-293T cells (A) and transiently transfected Ly3 cells (B). Data were repeated three times. (C) The effect of the overexpression of WT STAT3α^WT and mutant STAT3α^M206K on cell proliferation in the presence or absence of JAK2 inhibitor TG101348. Bars represent mean ± SD from 4 replicates. The experiment was repeated two times (**P = 0.0023). The data were analyzed by the two-tailed unpaired Student's t test.