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. 2013 Jul 4;8(7):e67789. doi: 10.1371/journal.pone.0067789

Figure 5. Gain and loss of Notch activity affects Dpp signaling in an opposite manner.

Figure 5

A. In heph(m-z-) embryos that manifest gain of Notch activity, the dorsal amnioserosa expands at the expense of the lateral regions. B. Expression of Dpp is up regulated in heph(m-z-) embryos. C. While embryos that manifest loss of Notch activity (N(m-z-,+), SuH(m-z-,+)) express lower levels of MAD, embryos that manifest gain of Notch activity (heph(m-z-,+)) express a higher level of MAD. Extracts from stage 0–8 embryos are shown. The same number of embryos was used for protein extraction and the same amount of the extract was loaded in each lane of a blot. The same samples were separated in 10% and 7.5% percentage gels for probing with MAD and Hsp 70 antibodies. Immuno-labeling experiments were repeated two times and most embryos at a particular stage showed the same ohenotype. The western blotting experiments wre repeated three times.