Figure 6. Notch activity promotes the expression of Pkc98E proteins in embryos.

A. Notch gain of function N^nd1 embryos express higher levels of both the predicted forms of Pkc98E (PA and PB). The same blots probed for Cactus reveal that a high level of Pkc98E is associated with high levels of P-Cactus. The same number of embryos was used for all samples and the same amount of extract was loaded in each lane. B. heph⁰³⁴²⁹ embryos with gain in Notch activity express high levels of PKCδ in the lateral regions. Embryos shown are ∼ stage 14. Both the western blotting and the immuno-labeling experiments were repeated twice.