Figure 9. The effects of Pkc98E knock down in embryos.

A. RNAi knock down of Pkc98E gene expression (short inverted repeat: PKCi 0174; long repeat: 2470) reduces the expression of Pkc98E proteins forms PA and PB and MAD. Extracts from stage 0–16 embryos are shown. The same sample was used for the two blots. * = background bands that additionally indicate protein loading is not an issue. B. Pkc98E knock down by RNAi reduces the level of P-Cactus and increases the level of Dorsal and Notch. Extracts from stage 0–16 embryos are shown. The same samples were used in the three blots. C. The level of the truncated Cactus form Cactus* is increased in PKCi embryos. Extracts from 8–16 stages (first two lanes) and 0–8 stages (last two lanes) are shown. The top blot based on 7.5% gel clearly shows P-Cactus and Cactus. These two bands merge in the 10% gel required to retain Cactus* (bottom blot). PKCi 0174 embryos show a similar response. In all the experiments, the same number of embryos was used for all samples and the same amount of the sample was loaded in each lane of a blot. Each result in these experiments was verified by at least three repetitions.