Figure 10. Pkc98E RNAi embryos manifest loss of Cactus and Dpp phenotypes.

A. Nuclear Dorsal expression in the ventral region of Pkci embryos is irregular and expanded. Irregularity is apparent even at the very initial stages of dv axis formation (top row) and becomes pronounced at later stages (middle row). The last row shows embryos from a lateral perspective. B. Pkci embryos manifest denticles in the normally naked lateral regions and loss of head and filzkorpor structures that are associated with loss of Cactus and Dpp functions. The phenotypes were verified in two independently generated embryos for each of the two Pkci lines used.