Figure 11. Loss of Pkc98E expression results in suppression of neurogenesis in the ventral region of embryos.

A. RNAi knock down of Pkc98E expression (da/Pkci2470) significantly reduces not only the number of Hunchback expressing cells (i.e., neuronal cells) but also the amount of Hunchback in the few cells expressing it (i.e., reduced neuronal potential). The Pkci0174 line gave similar results. Embryos shown are at stage 9. B. Embryos homozygous for the null allele Pkc98^Ef06221 (Pkc98E⁻) also manifest reduced number of Hunchback expressing cells and reduced amount of Hunchback in cells. Embryos shown are at stage 10. C. The production of excess neuronal cells in the ventral region of zygotic Notch null embryos (N⁻) is blocked when embryos are simultaneously homozygous for the Pkc98^Ef06221 null allele (N⁻; Pkc98E⁻). Embryos shown are at stage 11. The phenotypes in A were verified in two independently generated embryos for each of the two Pkci lines used (the two alleles showed different penetrance and expressivity), the phenotypes in B were verified by most embryos at stage 10, and the phenotypes in C was verified in two independent embryo samplings from the same cross.