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. 2013 Jul 4;8(7):e67846. doi: 10.1371/journal.pone.0067846

Figure 2. In vitro effects of miR-196b over-expression.

Figure 2

A) Relative viability of ME-180 and SiHa cells were assessed at 48 and 72 hours post-transfection with pre-miR-196b (30 nmol/L), compared to Negative Control pre-miR (30 nmol/L), using the Trypan Blue assay. B) Clonogenicity of ME-180 and SiHa cells were assessed by transfection with 30 nmol/L of pre-miR 196b or Negative Control pre-miR. At 48 hours post-transfection, cells were harvested then counted and re-seeded at low density in 6-well plates. After 10 days of incubation, cells were fixed and stained and the number of colonies (>50 cells) were counted. C) Relative proliferation of ME-180 and SiHa cells were examined at 24, 48, and 72 hours post-transfection with pre-miR-196b (30 nmol/L), compared to Negative Control pre-miR (30 nmol/L), using the MTS assay. D) Representative images (left) and histograms (right) depicting migratory ability (top) and invasiveness (bottom) of ME-180 cells that were transfected with 30 nmol/L of pre-miR 196b or Negative Control pre-miR, harvested at 24 hours post-transfection, then counted and re-seeded in the invasion chambers. Cell migration (top), and invasion (bottom), assessed at 48 hours after seeding in transwell chambers. All data represent the mean ± SEM from 3 independent experiments. NC, pre-miR Negative Control; *P<0.05; **P<0.01.