Table 1. Effect of PbCl2 on the relative amplitude (A) and half-life time (t1/2) of the exponential decay components of Chl a fluorescence yield after a single turnover flash measured in thylakoid membranes in the absence and in the presence of 50 µM DCMU.
Without DCMUPbCl2 (µM) | Fast Phase | Middle Phase | Slow Phase | |||
t1/2 (±10 µs) | A (±5%) | t1/2 (±0.3 ms) | A (±3%) | t1/2 (0.4 s) | A (±2%) | |
0 | 430 | 69 | 6.1 | 22 | 5.9 | 9 |
10 | 690 | 61 | 7.5 | 26 | 7.1 | 13 |
100 | 987 | 45 | 11.3 | 35 | 12.1 | 20 |
1000 | 1440 | 28 | 16.8 | 43 | 15.6 | 29 |
2000 | 1590 | 19 | 18.2 | 46 | 17.2 | 35 |
With DCMU PbCl2 (µM) | Fast Phase | Middle Phase | Slow Phase | |||
t1/2 (±5 ms) | A (±4%) | t1/2 (±0.03 s) | A (±3%) | t1/2 (±0.3 s) | A (±2%) | |
0 | 298 | 41 | 1.79 | 47 | 18.5 | 12 |
10 | 1130 | 26 | 1.92 | 53 | 22.1 | 21 |
100 | 1560 | 19 | 2.25 | 58 | 23.6 | 23 |
1000 | – | – | 4.35 | 68 | 31.2 | 32 |
2000 | – | – | 4.86 | 69 | 31.9 | 31 |