Figure 5. The impact of tissue- and cell-specific sex conversion on sexual conditioning.

(A) tra-1(gf) was expressed in all neurons by the H20 promoter or in ASE neurons by the gcy-5 and gcy-7 promoters to feminize the respective neurons of males, which were conditioned with the WT hermaphrodites. Males with feminized neurons are not attracted to NaCl after being conditioned with hermaphrodites. (B) Overexpression of fem-3(+) under the control of the H20 promoter to masculinize all neurons. The transgenic hermaphrodites were conditioned in a high-density population, but this procedure does not cause sexual conditioning. **, P<0.001; n.s., not significant. Error bars represent SEM. n = 56–64 animals for each condition.