Figure 6. Nitric oxide production in RAW 264.7 cells 6 hpi.

NO levels in the supernatants of RAW 264.7 cells were measured using the Griess assay. Levels in cells treated with MG132 (10 µM) were lower than untreated cells but there was no significant difference between any of the uninfected samples or controls. This decrease in NO production upon MG132 addition indicated that the increase in S-nitrosylation of caspase-3 was not due to increased NO production in response to infection. Experiments were repeated at least three times in triplicate and data from a representative experiment is shown.