Figure 8. Recovery of bacteria from RAW 264.7 cells up to 30 days post infection.

RAW 264.7 cells were infected as described and maintained in 3% FCS for a period of 30 days with regular media changes and maintenance of gentamycin at 50 µg/ml. Intracellular bacteria were harvested as before by lysing RAW 264.7 cells. Experiments were repeated at least three times in triplicate and data from a representative experiment is shown. Data was analyzed by an unpaired Student’s t-test. Statistically significant relationships are denoted. P value ***<0.005.