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. 2013 Jul 4;8(7):e67712. doi: 10.1371/journal.pone.0067712

Figure 6. Effects of adiponectin on Wnt signaling components of 3T3-L1 cells.

Figure 6

mRNA levels of the Wnt ligand family (A), Wnt receptors (B), and Fabp4 (C) in 3T3-L1 cells cultured with 50 (open bars), 150 (dotted bars), and 300 µg/ml (closed bars) of adiponectin shown as ratios to those of 3T3-L1 cells without adiponectin supplementation (n = 3, each). Means and SD. *p<0.05, **p<0.01. Protein levels of total and phosphorylated forms of CaMKII (D) and JNK (H) and relative protein levels of p-CaMKII (E, F, G) and p-JNK (I, J, K) in 3T3-L1 cells incubated with Wnt5a (open bars), Wnt5a and 50 (E, I), 150 (F, J), or 300 µg/ml (G, K) of adiponectin (dotted bars), or 50 (E, I), 150 (F, J), or 300 µg/ml (G, K) of adiponectin alone (hatched bars) compared with non-treated 3T3-L1 cells. The dose-response experiments were carried out independently at each concentration. Western blot analyses were performed twice with essentially the same results. Dots indicate individual data points (E, F, G, I, J, K). Ad, adiponectin.