Table 2. Primer sets to amplify the regions containing ndh genes.
| Chloroplast region | Primer name | Gene | Sequence | TM | Position | Product size(Elodea) | Product size (Najas) | Missing genes (Najas) | |
| LSC-Leu | tRNA-Leu-1F | tRNA-Leu(UAA) | Forward | TACACGGCCAAGGAATCTCG | 59.8 | 51740 | >2400 | 831 | ndhJ, ndhK, ndhCΨ |
| tRNA-Leu-1C-F | tRNA-Phe(GAA) | Forward | AGGGTCGAGTCAGGATAGCT | 59.4 | 51960 | >2100 | 611 | ||
| tRNA-Leu-1R | ndhC | Reverse | GGGTATTTGTCACCGGGGTT | 60 | 52570 | ||||
| IR b_SSC | NF_IRb_SSC_3F | past ycf1 | Forward | CTTCGGAAGAAATCCATTGGGCAAAAA | 57.02 | 119590 | ? | 324 | ndhF |
| NF_IRb_SSC_3R | rpl32 | Reverse | TGGAACTGCCATTTCAAAGCGACT | 57.58 | 119914 | ||||
| SSC_rpl32 | NF_SSC_rpl32_1F | rpl32 | Forward | TTGGCTGCGGTTAAAGCTTTTTCTTT | 57.02 | 119977 | >1350 | 1269 | none |
| NF_SSC_rpl32_1R | ccsA | Reverse | CCACTGCGACTGTAGAGCAGGC | 59.55 | 121246 | ||||
| SSC_ccsA | NF_SSC_ccsA_1F | ccsA | Forward | GCCTGCTCTACAGTCGCAGTGG | 59.55 | 121225 | >2400 | 1688 | ndhDΨ |
| NF_SSC_ccsA_1R | psaC | Reverse | TGCTTCCGCGCCAAGAACAGA | 59.32 | 122913 | ||||
| SSC_psaC | NF_SSC_psaC_1F | psaC | Forward | GTCCTCTGTTCTTGGCGCGGA | 59.13 | 122889 | >6400 | 1763 | ndhEΨ, ndhG, ndhI, ndhA, ndhHΨ |
| NF_SSC_psaC_1R | rps15 | Reverse | CGAAGGATTTTAGGAAAACGCCAACG | 57.58 | 124652 |
Ψ
pseudogene.
Primers are transferable among different Najas species.