Figure 9. Binding of insulator proteins to the eye enhancer, promoter, white gene and gypsy insulators in the transgenic construct.

Chromatin was isolated from transgenic flies carrying the construct or its derivatives described in Figure 8 was treated with antibodies against CP190, Mod(mdg4)-67.2 (designated as MOD), E(y)2, and Su(Hw). The results of ChIP are presented as a percentage of input DNA normalized relative to the endogenous positive binding site for insulator proteins from the 62D region. Relative locations of primers for ChIP are indicated under the construct scheme. Proximal and distal gypsy insulators are designated as Gp and Gd correspondingly. The rpl32 and tubulin (tub) coding regions were used as controls devoid of binding sites for the test proteins. Error bars indicate standard deviations of quadruplicate PCR measurements. Other designations are as in Figure 1.