Figure 10. Binding of Zeste to the white enhancer, promoter, and coding region in the transgenic constructs.

Results of immunoprecipitation experiments with chromatin isolated from transgenic flies and treated with anti-Zeste antibodies. (A) The results of ChIP (percentages of input DNA normalized relative to the endogenous positive binding site for Zeste from the Ubx promoter region) of specified chromatin regions with antibodies to Zeste in the transgenic construct in the presence or absence of gypsy insulator (Gy) in one copy. (B) The results of ChIP with antibodies to Zeste in the derivatives of the transgenic construct described in Figure 8 in the presence or absence of the gypsy insulator (Gy) in one copy. (C) Comparison of the results of ChIP with anti-Zeste antibodies in the presence of two copies of either gypsy or Fab-7 insulators (I, black boxes). Designations: E (the eye enhancer), P (promoter) and W (coding region) of the white gene. The rpl32 and tubulin (tub) coding regions were used as controls devoid of Zeste binding sites. Error bars indicate standard deviations of quadruplicate PCR measurements. Other designations are as in Figure 1 and 9.