Table 1. Genes with expression differences of 1.5-fold or greater in ΔmamA or complemented strains compared to the wildtype parent (H37Rv) in aerobic growth conditions.
Log2 expression ratio | p a | |||||
Gene | Symbol | ΔmamA/wildtype | ΔmamA::mamA/wildtype | Raw | FDRb | Distance (bp) to upstream MamA sitec |
Rv3263 | mamA | −4.05 | 2.51 | 2.0×10−12 | 8.2×10−9 | 3213 |
Rv0142 | −1.32 | −0.16 | 4.0×10−6 | 4.2×10−3 | 143 | |
Rv1239c | corA | −0.80 | −0.22 | 0.037 | 1.0 | 43 |
Rv3197A | whiB7 | −0.75 | 0.02 | 0.00067 | 0.31 | 336 |
Rv3083 | −0.72 | −0.19 | 0.0049 | 0.97 | 8 | |
Rv0102 | −0.72 | 0.03 | 0.0011 | 0.41 | 3 | |
Rv3085 | −0.68 | −0.12 | 0.011 | 1.0 | 8d | |
Rv3084 | lipR | −0.62 | −0.06 | 0.026 | 1.0 | 8d |
Rv3378c | −0.59 | −0.07 | 0.038 | 1.0 | 1067 | |
tRNA-pro | proU | −0.15 | −1.91 | 0.00034 | 0.24 | 2480 or 6630e |
Rv2463 | lipP | 0.016 | −2.62 | 8.18×10−9 | 1.7×10−5 | 2544 or 476e |
tRNA-glyf | glyV | −0.0089 | −2.66 | 2.54×10−7 | 0.00035 | 2430 |
ANOVA.
Method of Benjamini and Hochberg.
Distance from start of ORF to nearest upstream MamA site.
Distance from start of first ORF in operon (Rv3083) to nearest upstream MamA site.
Distances in wildtype/ΔmamA and complemented strains, respectively; complementation vector integrates in this region.
Complementation vector integrates in this gene.