Figure 1. Socs3fl/fl LysM cre mice show higher susceptibility to infection with M. tuberculosis.

Socs3^fl/fl LysM cre and Socs3^fl/fl littermate controls were sacrificed at indicated time points after aerosol infection with M. tuberculosis and colony forming units (CFU) per lung (A) and spleen (B) were assessed. The CFU per lung of individual mice and the median per group (n≥4) at the indicated time points after infection are depicted. Differences in CFU are significant (*p<0.05 and **p<0.01 Mann Whitney U test).Gross-pathology photograph of the lungs from Socs3^fl/fl and Socs3^fl/fl LysM cre mice 8 weeks after infection with M. tuberculosis (C). Histopathological scoring of hematoxylin-eosin stained paraffin lung sections from Socs3^fl/fl LysM cre and Socs3^fl/fl mice measured 4 weeks after infection with M. tuberculosis (D). The mean % lung area with granulomas or free of lesions ± SEM is displayed. Differences with controls are significant (n = 8 per group, *p<0.05 Student t test). The cumulative mortality of Socs3^fl/fl and Socs3^fl/fl LysM cre mice (n = 10) after aerosol infection with M. tuberculosis is depicted (E). Survival curves are different (Log-rank test p<0.005). CFU per lung, spleen and liver in Socs3^fl/fl and Socs3^fl/fl LysM cre mice (n≥5 per group) were assessed 6 weeks after infection with 10⁶ BCG i.v. (F). The median CFU and interquartile range per group are depicted. Differences in CFU are significant (**p<0.01 Mann Whitney U test). The mean percentage of Gr1+F4/80- neutrophils in the lung of Socs3^fl/fl and Socs3^fl/fl LysM cre mice (n = 5 per group) 3 weeks after infection with M. tuberculosis ± SEM was determined by FACS analysis (G). The accumulation of myeloid peroxidase (Mpo) transcripts in lungs from mice at 0, 3 or 8 weeks after M. tuberculosis infection (n≥5 per group) was determined by real time PCR. The mean fold Mpo mRNA increase ± SEM in is depicted (H).