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. 2013 Jul 4;9(7):e1003465. doi: 10.1371/journal.ppat.1003465

Figure 7. M029 blocks early activation of PKR signaling in MYXV-infected cells.

Figure 7

A) HeLa cells were left uninfected (M) (lanes 1 and 8) or infected with vMyx-GFP (lanes 2 to 7) or vMyx-M029ID (lanes 9 to 14) at an MOI of 3. Cells were collected at 2 (lanes 2 and 9), 4 (lanes 3 and 10), 8 (lanes 4 and 11), 12 (lanes 5 and 12), 24 (lanes 6 and 13) and 24a in the presence of AraC (lanes 7 and 14) h p.i. The membranes were first probed with anti phospho PKR (pPKR) antibody, stripped and probed for PKR and actin (loading control). B) Western blots showing expression of pPKR, PKR and actin in vMyx-M029ID infected samples collected after 2, 3, 4, 6, 8, 12, 24 hrs and 24a in the presence of AraC from HeLa cells. The relative level of phosphorylated PKR is indicated.