Figure 8. Transient knocking down of PKR by siRNA can rescue the replication of vMyx-M029KO and vMyx-M029ID.
A) HeLa and A549 cells were transiently transfected with control siRNA (panels in rows 1 and 3) or siRNAs targeting human PKR (final concentration of 100 nM) (panels in rows 2 and 4) for 48 h before infection with vMyx-GFP (column 1), vMyx-M029KO (column 2) and vMyx-M029ID (column 3) at an MOI of 3 for 48 h. B) Western blot of siRNA transfected cell lysates showing the level of PKR knock down in HeLa (Lane 2, panel 1) and A549 cells (lane 4, panel 3).