Skip to main content
. 2013 Jul 4;7(7):e2296. doi: 10.1371/journal.pntd.0002296

Figure 3. RVFV enrichment with NT53 incubation.

Figure 3

A) RVFV was spiked into cell culture media (DMEM+++) at various concentrations from 1.0E+5 to 1.0E+1 pfu/ml. NT53 was added to 1 ml of media and captured according to standardize protocols. The pellet was washed 4 times with water, followed by processing with Ambion's MagMax 96-well Viral RNA extraction kit. RVFV-spiked media without NT53 were processed in parallel. Viral RNA was quantitated by qRT-PCR with viral specific primers. B) RVFV was spiked into 100% bovine, sheep, and donkey serum at 1.0E+5 pfu/ml. NT53 were added to 1 ml of serum and captured according to a standardize protocol. The pellet was washed 4 times, followed by processing with Ambion's MagMax 96-well Viral RNA extraction kit. Serum without NT53 was processed in parallel. Viral RNA was quantitated by qRT-PCR.