Table 2. Melting temperatures of WT and mutant FbFP.

FbFP	T m(Celsius)	FbFP	T m(Celsius)
WT	42.8±0.3	N107F	50.9±0.3
H22K	45.3±0.2	N107Y	52.0±0.5
H22W	49.4±0.2	D109E	46.8±2.0
V25I	46.6±0.5	M111F	56.5±1.0
T30M	47.3±0.4	V120I	54.2±0.9
A33Y	46.6±0.3	N124F	50.2±0.1
T50M	36.4±1.7	N124Y	63.8±0.1
T54Y	43.7±0.1	N107F-N124F	59.3±0.8
A81M	39.8±2.3	N107Y-N124Y	69.5±0.1
V88L	41.0±2.5	N107Y-V120I	56.4±0.6
V90I	33.6±1.1	N107Y-N124Y-H22W	67.5±0.2
L106M	N/Aa	N107Y-N124Y-M111F	74.9±0.2

^a T _m was not determined due to the weak fluorescence of the sample at room temperature.