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. Author manuscript; available in PMC: 2013 Jul 4.
Published in final edited form as: Methods Enzymol. 2011;490:71–92. doi: 10.1016/B978-0-12-385114-7.00004-0

Table 1.

List of antibodies for detecting ER stress markers

Antibody Source Weight (kDA) Supplier Blocking buffer, Dilution
IRE1α Rabbit 130 Cell Signaling, #3294 BSA, WB 1:1000
Phospho-IRE1α Rabbit 110 Novus, NB100-2323 Milk, WB 1:1000
Spliced XBP-1 Rabbit 54 Santa Cruz, sc-7160 Milk, WB 1:1000
Total PERK Rabbit 150 Rocklan, 100-401-962 Milk, WB 1:1000
Phospho-PERK Rabbit 170 Cell Signaling, #3179 BSA, WB 1:1000
eIF2α Rabbit 36 Santa Cruz, sc-11386 Milk, WB 1:1000
Phospho-eIF2α Rabbit 38 Cell Signaling, #3597 BSA, WB 1:1000
ATF6α Rabbit 90 Santa Cruz, sc-22799 Milk. WB 1:100
Cleaved ATF6α Mouse 50 Imgenex, IMG-273 Milk, WB 1:1000
CHOP/GADD153 Mouse 31 Pierce, MA1-250 Milk, WB 1:2000
BiP/GRP78 Rabbit 78 Stressgen, SPA-826 Milk, WB 1:1000
IF: 1:100
ATF4 Rabbit 39 ProteinTech, 10835-1-AP Milk, WB 1:1000
PDI Mouse 58 Stressgen, SPA-891 Milk, WB 1:1000
IF: 1:100